Thermo Fisher Scientific BAFF Polyclonal Antibody, PeproTech

Applications and Tested Dilution

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Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography employing an immobilized Human BAFF matrix.

Sandwich ELISA:
To detect Human BAFF by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BAFF (500-P163BT) as a detection antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human BAFF.

Western Blot:
To detect Human BAFF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human BAFF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

BAFF is a membrane-bound cytokine expressed by B cell lineage cells. The soluble form binds to receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR.
It is a potent B cell activator, important for proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and promotes T cell activation and survival. Isoforms representing alternatively spliced and glycosylated variants have been identified.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.