Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), PE-eFluor 610, eBioscience

Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: ≤ 0.5 µg/test
• Publications: View 12 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• Brilliant Violet 480
• Brilliant Violet 650
• eFluor 450
• eFluor 660
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4), a 52 kDa transcription factor involved in B cell, T cell, and macrophage function.

• B cells: Highly expressed in mature plasma cells; regulates differentiation and transcription of B cell-specific genes (e.g., Prdm1/Blimp1).
• T cells: Implicated in Treg, Th2, Th9, and Th17 cell development and function; upregulated upon activation.
• Macrophages: Involved in polarization and regulation.
• Disease relevance: Upregulated in various hematologic malignancies.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Validated using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) according to BestProtocols® “Protocol B: One-step protocol for nuclear intracellular proteins”.
Use ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize concentration empirically.

Fluorophore Information:

• Excitation: 488–561 nm
• Emission: 607 nm
• Recommended filter: 610/20 band pass (PE-Texas Red equivalent)
• Compatible lasers: Blue, Green, Yellow-Green
• Light sensitivity: Protect from light (photo-oxidation sensitive)

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)
  Performance after fixation should be verified empirically.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The IRF (interferon regulatory factor) family consists of transcription factors with a unique tryptophan pentad repeat DNA-binding domain. IRFs regulate interferon responses to viral infection and control interferon-inducible genes.
IRF4 is lymphocyte-specific and negatively regulates toll-like receptor (TLR) signaling, central to innate and adaptive immunity. A chromosomal translocation t(6;14)(p25;q32) involving IRF4 and IgH loci is associated with multiple myeloma. Alternatively spliced transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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