Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), PE-eFluor 610, eBioscience

Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), PE-eFluor 610, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.5 µg/test
  View 27 publications

Product Specifications

Additional Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 661 / 737
• Brilliant Violet 421 / 480 / 650 / 786
• eFluor 450
• FITC
• Functional Grade
• PE / PE-Cyanine5 / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Request custom conjugation

Product Specific Information

The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils.
Ly-6G plays a role in neutrophil infiltration, recruitment, and migration, and associates with B2 integrins CD11a/CD11b.
Unlike RB6-8C5, 1A8-Ly6g binds only to Ly-6G on neutrophils and can be used for neutrophil depletion.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse bone marrow cells, ≤0.5 µg/test (100 µL final volume). Optimize antibody titration empirically.

Fluorophore Information:
PE-eFluor® 610 is excited at 488–561 nm and emits at 607 nm. Recommended filter: 610/20 band pass (PE-Texas Red® equivalent).
Sensitive to photo-induced oxidation — protect from light.

Fixation:
Samples may be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.

Laser Compatibility:
Excitation: 488–561 nm
Emission: 607 nm
Compatible Lasers: Blue, Green, Yellow-Green

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Ly6G (Lymphocyte antigen 6 complex locus G6D)

• Component of myeloid differentiation antigen Gr-1 (with Ly6C)
• Molecular weight: 21–25 kDa
• Expressed by myeloid-derived cells in bone marrow
• Marker for neutrophils, monocytes, and granulocytes
• Correlates with granulocyte differentiation and maturation
• Involved in anti-tumor response and local immune regulation

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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