
Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), PE-eFluor 610, eBioscience
마우스 Ly-6G(Gr-1) 항원을 인식하는 단클론 항체로, 중성구 검출 및 분석에 적합합니다. PE-eFluor 610 형광체로 결합되어 488–561 nm에서 여기되고 607 nm에서 방출됩니다. 유세포 분석에 최적화되어 있으며, 0.5 µg/test로 사용 권장됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), PE-eFluor 610, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8-Ly6g |
| Conjugate | PE-eFluor™ 610 |
| Excitation / Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574678 |
Additional Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 661 / 737
- Brilliant Violet 421 / 480 / 650 / 786
- eFluor 450
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436 / 600 / 702
- Request custom conjugation
Product Specific Information
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils.
Ly-6G plays a role in neutrophil infiltration, recruitment, and migration, and associates with B2 integrins CD11a/CD11b.
Unlike RB6-8C5, 1A8-Ly6g binds only to Ly-6G on neutrophils and can be used for neutrophil depletion.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse bone marrow cells, ≤0.5 µg/test (100 µL final volume). Optimize antibody titration empirically.
Fluorophore Information:
PE-eFluor® 610 is excited at 488–561 nm and emits at 607 nm. Recommended filter: 610/20 band pass (PE-Texas Red® equivalent).
Sensitive to photo-induced oxidation — protect from light.
Fixation:
Samples may be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
Laser Compatibility:
Excitation: 488–561 nm
Emission: 607 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D)
- Component of myeloid differentiation antigen Gr-1 (with Ly6C)
- Molecular weight: 21–25 kDa
- Expressed by myeloid-derived cells in bone marrow
- Marker for neutrophils, monocytes, and granulocytes
- Correlates with granulocyte differentiation and maturation
- Involved in anti-tumor response and local immune regulation
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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