Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), PE-eFluor 610, eBioscience
인간 및 마우스 c-Maf 단백질을 인식하는 sym0F1 단클론 항체로, PE-eFluor 610 형광표지형입니다. Flow Cytometry용으로 최적화되어 있으며, IL-4, IL-21 등 Th 세포 관련 인자 연구에 적합합니다. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (1.0 µg)/test
- View 4 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published species | Not Applicable |
| Host/Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | sym0F1 |
| Immunogen | Full-length E. coli-produced c-Maf (target family: c-MAF, UniProt ID: O75444-2, antigen range: 1–373) |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565/606 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848502 |
Available Conjugate Formats
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 480
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems.
It is crucial for IL-4 expression in Th2 cells and plays roles in Th17 and Tfh cell differentiation by regulating IL-21, IL-23R, and CXCR5 expression.
- Cross-reactivity: Does not cross-react with MafA or MafB.
- Applications Reported: Intracellular staining followed by flow cytometric analysis.
- Applications Tested: Pre-diluted and validated using Th17-polarized mouse splenocytes with Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
- Recommended Use: 5 µL (1.0 µg) per test (final volume 100 µL; 10⁵–10⁸ cells/test).
- Excitation/Emission: 488–561 nm / 607 nm.
- Recommended filter: 610/20 band pass (equivalent to PE-Texas Red).
Light sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57).
Target Information
c-Maf is a basic leucine zipper transcription factor and the cellular counterpart of oncogenic v-maf.
It regulates IL-4 expression in Th2 cells, crystalline gene expression in lens fibers, and insulin gene transcription in pancreatic islets.
c-Maf modulates Th2 differentiation and hematopoietic lineage specification and acts as a transcription factor for IL-10 expression in macrophages.
Chromosomal aberrations involving maf are associated with certain forms of multiple myeloma, and it is expressed in myeloma cell lines and monocytes/macrophages.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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