Thermo Fisher Scientific pro BDNF Polyclonal Antibody
Rabbit polyclonal antibody recognizing pro BDNF. Validated for WB and IHC (Paraffin). Reacts with human, mouse, and rat. Lyophilized form for stable storage. Suitable for neuroscience and neurotrophin research.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from mouse pro BDNF conjugated to blue carrier protein was used as the antigen |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: pro BDNF.
Target Information
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. It is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature 14 kDa protein, pro-BDNF is biologically active and secreted in synaptic vesicles along with the mature form.
BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, though binding to the lower-affinity receptor p75-NTR has also been demonstrated. BDNF promotes neuronal survival and differentiation and plays a critical role in memory formation and synaptic regulation.
Expression of BDNF is reduced in both Alzheimer’s and Huntington’s disease patients and may also influence stress response and mood disorder biology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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