Thermo Fisher Scientific proBDNF Polyclonal Antibody
Rabbit polyclonal antibody recognizing proBDNF for use in WB and IHC. Validated in rat samples. Lyophilized form, reconstitutable with distilled water. Suitable for neuroscience research on BDNF signaling and neurotrophin pathways.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 10–50 µg/mL | - |
Product Specifications
| Specification | Detail |
|---|---|
| Published species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from mouse proBDNF conjugated to blue carrier protein was used as the antigen. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10717050 |
Product Specific Information
Reconstitute with 500 µL of distilled water.
Target Information
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. It is synthesized as pre-proBDNF, then cleaved to proBDNF. Although further processing generates the mature 14 kDa protein, proBDNF is biologically active and secreted in synaptic vesicles along with the mature form.
BDNF is widely expressed in the central nervous system and acts on several classes of neurons via autocrine and paracrine signaling. The main receptor is TrkB, though it can also bind to the lower-affinity receptor p75-NTR.
BDNF promotes neuronal survival and differentiation and plays a critical role in memory formation and synaptic regulation. Expression is reduced in Alzheimer’s and Huntington’s disease patients and may influence stress response and mood disorder biology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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