Thermo Fisher Scientific Themis Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Themis (N-terminal region) antibody was generated from a peptide that included amino acids in the N-terminal region of human themis. This sequence has high homology to the conserved region in rat and mouse themis.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2943005

Product Specific Information

The antibody detects a 72 kDa* protein corresponding to the molecular mass of themis on SDS-PAGE immunoblots of mouse spleen and human K562 cells.

Target Information

Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.