Thermo Fisher Scientific Phospho-TRPM7 (Ser1493) Polyclonal Antibody
Phospho-TRPM7 (Ser1493) 특이적 토끼 폴리클로날 항체로, 인간 TRPM7 단백질 인산화 형태를 검출합니다. Western blot에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제되었습니다. -20°C에서 보관하며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-TRPM7 (Ser-1493) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1493 in human TRPM7. This site is conserved in rat and mouse TRPM7, but the homology surrounding the site is low. This site has no homology to other TRPM family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2943006 |
Product Specific Information
The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1493) and then affinity purified using phospho-TRPM7 (Ser-1493) peptide.
This antibody detects a 220 kDa protein on SDS-PAGE immunoblots of human MDA-MB-231 cells stimulated with calyculin A and detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7.
These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.
Target Information
TRPCs are mammalian homologs of the Drosophila transient receptor potential (trp) protein and function as ion channels mediating capacitative calcium entry into the cell.
TRP-PLIK is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium.
As a kinase, it phosphorylates itself and other substrates. The kinase activity is necessary for channel function, as demonstrated by its dependence on intracellular ATP and by kinase mutants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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