Thermo Fisher Scientific Phospho-TRPM7 (Ser1569) Polyclonal Antibody
Phospho-TRPM7 (Ser1569) 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. -20°C 보관, 연구용 전용 제품입니다.
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Applications
- Western Blot (WB): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-TRPM7 (Ser-1569) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1569 in human TRPM7. This site is well conserved in rat and mouse TRPM7, and is conserved in TRPM6 (Thr-1724) however the site is threonine instead of serine. This site has no homology to other TRPM family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2943008 |
Product Specific Information
The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1569) and then affinity purified using phospho-TRPM7 (Ser-1569) peptide.
This antibody detects a 220 kDa protein on SDS-PAGE immunoblots of human MDA-MB-231 stimulated with calyculin A and rat PC12 cells, as well as a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7.
These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.
Target Information
TRPCs are mammalian homologs of the Drosophila transient receptor potential (trp) protein and function as ion channels mediating capacitative calcium entry.
TRP-PLIK is both an ion channel and a kinase, conducting calcium and monovalent cations to depolarize cells and increase intracellular calcium.
Its kinase activity is essential for channel function, dependent on intracellular ATP, and lost in kinase mutants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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