Thermo Fisher Scientific HDAC2 Monoclonal Antibody (3F3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

2.5 µg/10^6 cells

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3F3

Immunogen

Synthetic peptide, corresponding to amino acids 473-488 of Human HDAC2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HDAC2,uniProtId:Q92769-1,ncbiNodeId:9606,antigenRange:473-488,antigenLength:488,antigenImageFileName:MA5-18061_HDAC2_Q92769-1_House_mouse.svg,antigenImageFileNamePDP:MA5-18061_HDAC2_Q92769-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539444

Product Specific Information

Western blot results show a band at 55 kDa, which matches the predicted molecular weight.

This antibody is predicted to detect HDAC2 in Chicken and Bovine samples.

Target Information

HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.