Thermo Fisher Scientific HDAC2 Monoclonal Antibody (3F3)
HDAC2 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry, IP, ChIP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 1 mg/mL 농도의 액상 형태로 제공되며, Protein G로 정제됨. 연구용으로만 사용 가능.
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Thermo Fisher Scientific HDAC2 Monoclonal Antibody (3F3)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3F3 |
| Immunogen | Synthetic peptide corresponding to amino acids 473–488 of Human HDAC2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539444 |
Product Specific Information
Western blot results show a band at 55 kDa, matching the predicted molecular weight.
This antibody is predicted to detect HDAC2 in Chicken and Bovine samples.
Target Information
HDAC2 belongs to the histone deacetylase family. These enzymes act via large multiprotein complexes and remove acetyl groups from lysine residues on core histones (H2A, H2B, H3, and H4). HDAC2 forms transcriptional repressor complexes with various proteins, including the zinc-finger transcription factor YY1, playing a key role in transcriptional regulation, cell cycle progression, and development. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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