Thermo Fisher Scientific EAPP Monoclonal Antibody (1E4)
EAPP 단백질을 인식하는 Mouse monoclonal antibody (Clone 1E4). Western blot, ICC/IF, IP에 사용 가능. Human 및 Mouse 반응성. Protein G 정제, 1 mg/mL 농도의 액상 형태. 세포주기 조절 및 E2F1 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:200 | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E4 |
| Immunogen | N-terminal fragment of human EAPP (sequence: 1–266) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539445 |
Target Information
This protein may play an important role in the fine-tuning of both major E2F1 activities — the regulation of the cell cycle and the induction of apoptosis. It promotes S-phase entry and inhibits p14(ARP) expression.
The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins, and is also a target of transforming proteins of small DNA tumor viruses.
E2F proteins contain conserved domains including:
- DNA binding domain
- Dimerization domain (for interaction with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain (within the transactivation domain)
The C-terminal of EAPP binds the N-terminal of E2F1 and also interacts with E2F2 and E2F3, but not E2F4.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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