
Thermo Fisher Scientific TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), Super Bright 780, eBioscience
마우스 γδ TCR 복합체를 특이적으로 인식하는 eBioGL3 단클론 항체. Super Bright 780 형광체로 결합되어 유세포 분석에 최적화. 405 nm 레이저로 여기, 780 nm 방출. 광민감성 주의 및 Super Bright Staining Buffer 사용 권장.
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Thermo Fisher Scientific TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), Super Bright 780
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioGL3 (GL-3, GL3) |
| Conjugate | Super Bright™ 780 |
| Excitation / Emission Max | 413 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744918 |
Additional Formats
- Unconjugated
- APC
- Biotin
- Brilliant Violet 650
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 645
- Request custom conjugation
Product Specific Information
The GL3 monoclonal antibody reacts with the gamma delta T-cell receptor complex (TCR) on all γδ TCR-bearing T cells, but does not react with αβ TCR.
γδ TCR is found in thymus, epidermis, intestinal epithelium, peritoneal cavity, and lymphoid tissues.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes; ≤0.5 µg/test
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume.
Recommended careful titration for optimal performance.
Super Bright 780 Characteristics
- Tandem dye excited by violet laser (405 nm), emits at 780 nm
- Recommended filter: 780/60 bandpass
- Ensure instrument compatibility
Usage Recommendations
- When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (SB-4401) to reduce polymer interactions.
- Compensation values may differ between beads and cells; set compensation with stained cells when possible.
- Light sensitive: protect vial and samples from light.
- Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution.
Excitation / Emission / Laser
- Excitation: 405 nm
- Emission: 780 nm
- Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
TCR gamma/delta are specialized T cells essential for adaptive immune response.
They discriminate self and foreign peptides presented by MHC class II molecules.
Mutations or abnormal recognition are linked to autoimmune diseases and tumors.
Gamma-delta T cells are mainly found in epithelial tissues and exhibit antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures.
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