Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Super Bright 780, eBioscience
마우스 MHC Class II (I-A/I-E)를 인식하는 M5/114.15.2 클론 단일클론 항체로, Super Bright 780 형광체로 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, 높은 감도와 특이성을 제공합니다. 연구용으로 면역세포 분석 및 항원 제시 관련 연구에 적합합니다.
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Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Super Bright 780, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 36 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Mouse |
| Host/Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 780 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M5/114.15.2 |
| Conjugate | Super Bright™ 780 |
| Excitation/Emission Max | 413/780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2744916 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 496, 661, 737
- Brilliant Violet 421, 605, 650, 711, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue 510, 610-70S, 660-120S
- NovaFluor Red 685, 710
- NovaFluor Yellow 570, 590, 660
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702
- Custom conjugation available
Product Specific Information
The M5/114.15.2 monoclonal antibody reacts with mouse MHC class II (I-A and I-E) glycoproteins. It detects polymorphic determinants on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from specific mouse haplotypes (H-2 b, d, q, p, r, u).
- Applications Reported: Suitable for flow cytometric analysis.
- Applications Tested: Validated on stimulated mouse splenocytes at ≤0.25 µg/test (100 µL final volume).
- Recommended Use: Carefully titrate for optimal assay performance.
- Excitation: 405 nm; Emission: 780 nm; Laser: Violet.
Super Bright 780 Dye Information:
- Tandem dye excited by violet laser (405 nm), emits at 780 nm.
- Use 780/60 bandpass filter for optimal detection.
- Sensitive to photo-induced oxidation — protect from light.
- Store samples in IC Fixation Buffer or 1-step Fix/Lyse Solution up to 3 days at 4°C in dark.
- Use Super Bright Complete Staining Buffer (SB-4401) to minimize non-specific polymer interactions.
- Compensation values may vary depending on bead type; recommended to set compensation using cells.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
MHC class II molecules are transmembrane glycoproteins on antigen-presenting cells (macrophages, dendritic cells, B cells). They bind exogenous antigens and present them to T cells. The antigen-binding groove between alpha and beta chains is open-ended and accommodates peptides of 15–24 amino acids.
HLA and MHC antibodies are crucial in Immunopeptidomics, aiding in neoantigen identification and characterization via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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