Thermo Fisher Scientific CHD4 Monoclonal Antibody (3F2/4)
CHD4 단백질을 인식하는 Mouse IgG2a 모노클로날 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. 인간 및 생쥐 반응성. Protein A/G 정제, PBS 버퍼에 보존제 없음. 연구용으로만 사용.
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Thermo Fisher Scientific CHD4 Monoclonal Antibody (3F2/4)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1.0 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
| ELISA | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3F2/4 |
| Immunogen | Synthetic peptide (N- and C-terminal peptides used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ). The specific peptide recognized has not been tested. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The CHD4 gene product belongs to the SNF2/RAD54 helicase family and is a key component of the nucleosome remodeling and deacetylase (NuRD) complex. It plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis may develop antibodies against this protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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