Thermo Fisher Scientific CHD4 Monoclonal Antibody (3F2/4)
CHD4 단백질을 인식하는 마우스 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. Protein A/G로 정제된 액상 형태이며, 인간과 마우스 시료에 반응. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1.0 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
| ELISA | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3F2/4 |
| Immunogen | Synthetic peptide (N- and C-terminal peptides used for immunization: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ). The specific peptide recognized has not been tested. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The CHD4 gene encodes a protein belonging to the SNF2/RAD54 helicase family. It is a key component of the nucleosome remodeling and deacetylase (NuRD) complex, playing a critical role in epigenetic transcriptional repression. Autoantibodies against CHD4 are observed in patients with dermatomyositis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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