Thermo Fisher Scientific CHD4 Monoclonal Antibody (3F2/4)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

0.5-1 µg/mL

-

Flow Cytometry (Flow)

0.5-1 µg/10^6 cells

-

ELISA (ELISA)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

3F2/4

Immunogen

Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CHD4,uniProtId:Q14839-1,ncbiNodeId:9606,antigenRange:1894-1912,antigenLength:1912,antigenImageFileName:1108-MSM1-P0_CHD4_Q14839-1_House_mouse.svg,antigenImageFileNamePDP:1108-MSM1-P0_CHD4_Q14839-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.