Thermo Fisher Scientific CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), FITC, eBioscience
HIS51 클론의 CD90.1(Thy-1.1) 단클론 항체로, FITC로 표지되어 마우스 및 랫트 세포 분석에 적합. Flow cytometry에 최적화되어 있으며 0.06 µg/test로 사용 가능. 488 nm 여기, 520 nm 방출 특성. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunocytochemistry (ICC/IF) | - | View 4 publications |
| Flow Cytometry (Flow) | 0.06 µg/test | View 81 publications |
| Miscellaneous PubMed (Misc) | - | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Cat, Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS51 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465151 |
Additional Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule.
In rats, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflamed endothelia, and other cell types.
In CD90.1-expressing mouse strains (PL, AKR), CD90 is expressed by early hematopoietic cells in bone marrow, thymocytes, and mature T cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions.
Use ≤ 0.06 µg per test (100 µL final volume). Optimal performance should be determined by titration.
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90 (Thy-1) antigen is a GPI-linked glycoprotein member of the Immunoglobulin superfamily.
It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts.
Thy-1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other brain events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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