
Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), FITC, eBioscience
마우스 CD90.2(Thy-1.2)를 인식하는 단클론 항체로, FITC로 표지되어 흐름세포분석(Flow Cytometry)에 적합합니다. 53-2.1 클론이며 Rat IgG2a, kappa 아이소타입입니다. 0.5 mg/mL 농도의 액상 형태로 제공되며, 4°C 암조건에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 26 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-2.1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465153 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 615
- eFluor 450 / eFluor 506
- NovaFluor Blue 510 / 555 / 610-70S / 660-120S / 760
- NovaFluor Red 710
- NovaFluor Yellow 570 / 660
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- Super Bright 436 / 600 / 645 / 702
- Request custom conjugation
Product Specific Information
The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains (e.g., BALB/c, CBA, C3H, C57BL/6, C58/, SJL). Cells from CD90.1-expressing strains (e.g., PL, AKR) do not stain with 53-2.1.
CD90 is involved in T cell adhesion and signal transduction regulation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
- Excitation / Emission: 488 nm / 520 nm (Blue Laser)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90.2 (Thy-1) is a GPI-linked glycoprotein and a member of the immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts.
Thy-1 plays roles in cell-cell interactions during synaptogenesis and other neuronal events.
CD90.2 (18 kDa) is found in hematopoietic cells, neurons, connective tissue, fibroblasts, stromal cell lines, and tumor endothelial cells. In mice, it is expressed on all thymocytes and peripheral T lymphocytes.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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