Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (GL1), FITC, eBioscience
마우스 CD86(B7-2) 인식 단일클론 항체(GL1)로, FITC 형광 표지되어 Flow Cytometry에 최적화됨. 면역세포 활성화 및 공동자극 경로 연구에 활용 가능. 0.5 mg/mL 농도, 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 141 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GL1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465147 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 737
- Brilliant Violet 605
- Brilliant Violet 786
- eFluor 450
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated upon stimulation through BCR complex, CD40, and cytokine receptors. It is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells.
CD86 interacts with T cell surface molecules CD28 and CD152 (CTLA-4), playing a critical role in T-B crosstalk, T cell costimulation, and antibody production. The upregulation kinetics of CD86 support its major contribution during the primary immune response.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on stimulated mouse splenocytes at ≤0.125 µg/test (100 µL volume). Optimal titration recommended.
Excitation: 488 nm Emission: 520 nm Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD86 is one of two ligands (the other being CD80) for CTLA4 and CD28. It acts as a costimulatory molecule essential for T-cell activation during antigen presentation. Insufficient co-stimulation can induce immune tolerance. CD86 is a type I membrane protein of the immunoglobulin superfamily, expressed on antigen-presenting cells. It binds CD28 and CTLA-4 on T cells, respectively promoting or inhibiting T-cell activation. Alternative splicing produces multiple isoforms. CD86 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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