Thermo Fisher Scientific NTH1 Polyclonal Antibody
Human NTH1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, ELISA, IP에 사용 가능. 산화적 DNA 손상 복구 연구에 활용. 고순도 친화 크로마토그래피 정제, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N-epitope synthetic peptide taken within amino acid region 1–100 on human NTHL1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Oxygen radicals damage chromosomal DNA causing cell death and inducing mutations. Although much attention has focused on the repair of 8-oxo-G, many other damaged DNA sites are created by free-radical attack.
The human NTH1 repair protein acts on a wide range of oxidatively damaged DNA sites. As a homologue of E. coli Endonuclease III, NTH is a DNA glycosylase with apurinic/apyrimidinic lyase activity.
NTH protein has broad substrate specificity, including numerous ring saturation and fragmentation products of pyrimidines. Research indicates NTH plays a crucial role in removal of oxidative base lesions in mitochondrial DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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