Thermo Fisher Scientific NTH1 Polyclonal Antibody, FITC
Thermo Fisher Scientific의 NTH1 Polyclonal Antibody, FITC는 인간 NTHL1 단백질을 인식하는 토끼 IgG 항체로, WB, ELISA, IP에 사용 가능합니다. FITC 형광 표지로 498/517 nm 파장에서 검출되며, 산화적 DNA 손상 연구에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C-epitope synthetic peptide within amino acid region 190–290 on human NTHL1 protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Oxygen radicals damage chromosomal DNA, causing cell death and inducing mutations. Although much focus has been on the repair of 8-oxo-G, many other DNA lesions arise from free-radical attacks. The human NTH1 repair protein acts on a wide range of oxidatively damaged DNA sites. As a homologue of E. coli Endonuclease III, NTH is a DNA glycosylase with apurinic/apyrimidinic lyase activity. It shows broad substrate specificity, including ring-saturated and fragmented pyrimidine products. Research suggests NTH plays a crucial role in removing oxidative base lesions in mitochondrial DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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