Thermo Fisher Scientific Phospho-p130Cas (Tyr165) Polyclonal Antibody
p130Cas 단백질의 Tyr165 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot 및 Immunocytochemistry에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로, 단기 4°C, 장기 -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human p130Cas (phospho Tyr165). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.08 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2736396 |
Product Specific Information
- Positive Control: Neuro2A, C8D30, NIH-3T3, C2C12, Rat2
- Predicted Reactivity: Mouse (100%), Rat (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signaling via v-Crk and v-Src oncoproteins.
It has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich region.
p130Cas is implicated in various biological processes including cell adhesion, migration, growth factor stimulation, cytokine receptor engagement, and bacterial infection.
Physiological functions include cardiovascular development, actin filament assembly, and Src-induced cell transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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