Thermo Fisher Scientific SEMA6A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:250

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Semaphorin 6A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the cytoplasmic region of human Sema-6A. The sequence used has high homology to similar regions in rat and mouse Sema-6A, and has low homology to other semaphorin family members.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942989

Product Specific Information

The antibody detects a 120kDa* protein corresponding to the apparent molecular mass of Sema-6A on SDS-PAGE immunoblots of Sema-6A transfected COS7 cells, human colorectal cancer cells, and rat PC12 cells. In addition, shRNA knockdown of Sema-6A expression significantly reduces detection of Sema-6A using anti-Sema-6A (a.a. 772-787) antibody.

Target Information

SEMA6A can act as repulsive axon guidance cues. It may play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.