Thermo Fisher Scientific Ubiquitin Recombinant Rabbit Monoclonal Antibody (FK2), APC

Applications and Tested Dilution

Product Specifications

Available Formats

• FITC (Cat. No. MA5-45085)
• PerCP (Cat. No. MA5-45086)
• Custom conjugation available upon request

Product Specific Information

A 1:1,000 dilution of MA5-45084 was sufficient for detection of Ubiquitin in 2 µg of purified poly-ubiquitin chains (Poly-Ubiquitin: Ub1–Ub7 WT Chains K63-linked, K48-linked) by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Detects ubiquitinated proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.

Target Information

Ubiquitin is a highly conserved protein that plays a central role in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor consisting of either polyubiquitin chains or a single ubiquitin fused to another protein. The gene encodes a fusion protein with ubiquitin at the N-terminus and ribosomal protein S27a at the C-terminus.
Upon expression, post-translational processing generates free ubiquitin monomer and ribosomal protein S27a, a component of the 40S ribosomal subunit containing C4-type zinc finger domains.
Pseudogenes for this gene exist in the genome. Similarly, ribosomal proteins L40 and S30 are synthesized as fusion proteins with ubiquitin or ubiquitin-like proteins (fubi). Multiple alternatively spliced transcript variants encoding the same proteins have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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