Thermo Fisher Scientific HSP70 Monoclonal Antibody (C92F3A-5), APC
HSP70 단백질을 검출하기 위한 APC 결합 단클론 항체로, Western blot, IHC, ICC, 유세포분석 등 다양한 응용에 적합합니다. 사람, 생쥐 등 여러 종에 반응하며, 단백질 안정화 및 스트레스 반응 연구에 유용합니다.
- 카탈로그번호
- MA545090
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, C. elegans, Chicken, Fruit fly, Fish, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C92F3A-5 |
| Immunogen | Human HSP70 |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 95.46 mM phosphate / 2.48 mM MES with 2 mM EDTA |
| Contains | No preservative |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931544 |
Additional Formats
- FITC: MA5-45091
- PerCP: MA5-45092
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45090 was sufficient for detection of HSP70 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 70 kDa. Does not cross-react with HSC70 (HSP73).
Target Information
HSP70 is a heat shock protein expressed in response to various biological stresses, including high temperatures. The HSP70 family is highly conserved and induced by diverse stress conditions across organisms.
Human HSP70 family members include:
- HSP70: Strongly inducible and constitutively expressed in primate cells
- HSP72: Induced exclusively under stress conditions
- HSC70 (HSP73): Constitutively expressed, involved in clathrin-coated vesicle uncoating
- GRP78 (BiP): 78 kDa glucose-regulated protein localized in the endoplasmic reticulum
- HSP75 (p75): Mitochondrial protein
HSP70 stabilizes existing proteins against aggregation, assists folding of newly translated proteins, and participates in the ubiquitin-proteasome pathway through interaction with AU-rich element RNA-binding protein 1.
The HSP70 gene is located in the major histocompatibility complex (MHC) class III region, clustered with related genes encoding similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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