Thermo Fisher Scientific Ubiquitin Recombinant Rabbit Monoclonal Antibody (RGL3R), FITC
FITC 형광 표지된 Ubiquitin 재조합 토끼 단클론 항체로 다양한 생물 종에 반응합니다. Western blot 및 ELISA에 1:1,000 희석으로 사용 가능하며, 단백질 A 정제 및 보존제 무첨가. 4°C 암소 보관, 폴리유비퀴틴 검출에 적합.
- 카탈로그번호
- MA545082
- 판매단위
- pk
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Thermo Fisher Scientific Ubiquitin Recombinant Rabbit Monoclonal Antibody (RGL3R), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Human |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RGL3R |
| Immunogen | Ubiquitin conjugated lysozyme |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931536 |
Additional Formats
- APC (Cat. MA5-45081)
- PerCP (Cat. MA5-45083)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45082 was sufficient for detection of ubiquitin in 2 µg of purified poly-ubiquitin chains (Ub1–Ub7, K63-linked, K48-linked) by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Detects ubiquitinated proteins, including polyubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.
Target Information
Ubiquitin is a highly conserved protein involved in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein consisting of polyubiquitin chains or a single ubiquitin fused to another protein.
The gene encodes a fusion protein with ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. Ribosomal protein S27a, part of the 40S ribosomal subunit, contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene exist in the genome.
Similar fusion patterns are found in ribosomal proteins L40 and S30, which are synthesized with ubiquitin or ubiquitin-like proteins. Multiple alternatively spliced transcript variants encoding the same proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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