Thermo Fisher Scientific Desmin Monoclonal Antibody (DE-U-10), eFluor 660, eBioscience
Desmin 단백질을 인식하는 마우스 단클론 항체(DE-U-10)로, eFluor 660 형광 표지. 근육세포 및 근육 유래 종양 연구에 적합. IHC, ICC, Flow Cytometry 등 다양한 응용 가능. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific Desmin Monoclonal Antibody (DE-U-10), eFluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
| Flow Cytometry (Flow) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Chicken, Cat, Goat, Hamster, Human, Mouse, Sheep, Pig, Rabbit, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DE-U-10 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574355 |
Product Specific Information
Description:
The monoclonal antibody DE-U-10 recognizes desmin, a class-III intermediate filament protein predominantly found in skeletal, cardiac, and smooth muscle. Desmin plays a key role in muscle cell development, structure, and mitochondrial function. It forms scaffolds around the Z-disk of sarcomeres.
Desmin knockout mice show defects in muscle function and mitochondrial activity. Mutations in desmin are associated with desmin-related myopathy (DRM), cardiomyopathy dilated type 1I (CMD1I), and neurogenic scapuloperoneal syndrome (Kaeser syndrome).
The DE-U-10 antibody recognizes desmin from multiple species including human, mouse, rat, feline, bovine, goat, hamster, sheep, porcine, rabbit, and chicken. It also labels tumors derived from muscle tissue such as leiomyomas and rhabdomyosarcomas.
Applications Reported:
- Immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections
- Microscopy
- Immunocytochemistry
Applications Tested:
- IHC (P): ≤10 µg/mL using high pH antigen retrieval
- ICC/IF: ≤10 µg/mL on fixed and permeabilized cells
Titrate as needed for optimal performance.
Fluorophore Information:
eFluor™ 660 replaces Alexa Fluor™ 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Desmin (~53 kDa) shows high tissue specificity, expressed mainly in cardiac, skeletal, and smooth muscle. Its expression is regulated during muscle cell differentiation. In skeletal and cardiac muscle, desmin localizes to the Z-disk and intercalated disk. In smooth muscle, expression is more heterogeneous. Coexpression of vimentin and desmin is observed in muscle-derived tumors such as rhabdomyosarcomas and leiomyosarcomas. Altered desmin distribution is noted during myocardial dysfunction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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