Thermo Fisher Scientific COPII Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:200

View 10 publications 10 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Functional Assay (Functional)

-

View 1 publication 1 publication

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fruit fly, Hamster, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:COPII,uniProtId:Q5XFW8-1,ncbiNodeId:10114,antigenRange:1-18,antigenLength:322,antigenImageFileName:PA1-069A_COPII_Q5XFW8-1_Rabbit.svg,antigenImageFileNamePDP:PA1-069A_COPII_Q5XFW8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2301581

Product Specific Information

PA1-069A detects coatomer-protein II (COPII) from rat, mouse and human cells.

PA1-069A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~85 kDa protein representing COPII from PC-12 cell extract.

The PA1-069A immunogen is a synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII. This sequence is completely conserved between human, mouse, and rat. PA1-069A immunizing peptide (Cat. # PEP-127) is available for use in neutralization and control experiments.

Target Information

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta`-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits. COPII consists of three parts: Sar1p and the two protein complexes, Sec23p-Sec24p and Sec13p-Sec31p.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.