Thermo Fisher Scientific MINT3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) E F L P E P Q H P P G P P T M D L E(19) of rat Mint3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MINT3,uniProtId:O70248-1,ncbiNodeId:10114,antigenRange:2-19,antigenLength:569,antigenImageFileName:PA1-072_MINT3_O70248-1_Rabbit.svg,antigenImageFileNamePDP:PA1-072_MINT3_O70248-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2057069

Product Specific Information

PA1-072 detects munc-18 interacting protein 3 (Mint3) from mouse cells and rat brain samples.

PA1-072 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~86 kDa protein representing Mint3 from AtT20 cell extract and rat brain samples.

The PA1-072 immunogen is a synthetic peptide corresponding to residues M(1) E F L P E P Q H P P G P P T M D L E(19) of rat Mint3. The PA1-072 immunizing peptide (Cat. # PEP-135) is available for use in neutralization and control experiments.

Target Information

The munc-18 interacting protein (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint1 and 2 appear to be expressed exclusively in the brain and are found to bind to Munc18, an essential component of the synaptic vesicle fusion machinery. Mint3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that mint3 does not interact with munc-18. Mint3 has been found to interact with the Alzheimers Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that mint3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimers disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.