Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eBioscience

Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 510, 555, 610-70S, 660-120S, 760
• NovaFluor Red 710
• NovaFluor Yellow 570, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright 436, 600, 645, 702
• Request custom conjugation

Product Specific Information

The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains such as BALB/c, CBA, C3H, C57BL/6, C58/, and SJL. Strains expressing CD90.1 (e.g., PL and AKR) do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: Flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.06 µg/test (100 µL final volume). Optimal titration recommended.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD90.2 (Thy-1) antigen is a GPI-linked glycoprotein of the Immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts. Thy-1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other neural events. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic and neuronal cells, connective tissue, fibroblast and stromal cell lines, and tumor endothelial cell lines. In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.