Thermo Fisher Scientific CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), eBioscience
Rat 및 Mouse CD90.1(Thy-1.1)을 인식하는 HIS51 단클론 항체로, Flow Cytometry 및 IHC(Frozen)에 적합. 높은 순도(>90%) 및 낮은 응집도(<10%)를 보유하며, PBS buffer에 0.09% sodium azide 포함. 연구용으로만 사용 가능.
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Thermo Fisher Scientific CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 5 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 0.125 µg/test | 77 |
| Functional Assay | - | 1 |
| Miscellaneous PubMed | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS51 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467373 |
Available Conjugated Formats
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available upon request
Product Specific Information
The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule.
In rats, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, neurons, inflamed endothelia, and other cell types.
In CD90.1-expressing mouse strains (PL and AKR), CD90 is expressed by early hematopoietic cells, thymocytes, and mature T cells.
Applications Reported: Flow cytometric analysis and immunohistochemical staining of frozen tissue sections.
Applications Tested: Verified by flow cytometric analysis of rat thymocyte and splenocyte suspensions.
Recommended use ≤ 0.125 µg/test (100 µL final volume). Cell number can vary from 10⁵–10⁸ cells/test and should be empirically determined.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90 (Thy-1) is a GPI-linked glycoprotein and a member of the immunoglobulin superfamily.
It is expressed in murine T cells, thymocytes, neural cells, Kupffer cells, and fibroblasts.
Thy-1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other neural events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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