Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), eBioscience

Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Alexa Fluor 488
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 711
• Brilliant Violet 786
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700 / 725 / 755
• NovaFluor Yellow 610 / 690 / 700 / 755
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Custom conjugation available upon request

Product Specific Information

The IT2.2 monoclonal antibody reacts with human CD86 (B7-2), an ~80 kDa surface receptor belonging to the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated upon stimulation through the BCR complex, CD40, and cytokine receptors.

CD86 interacts with T-cell surface molecules CD28 and CTLA-4 (CD152), playing a critical role in T-B cell crosstalk, T-cell costimulation, and antibody production. Upregulation kinetics suggest its major role during the primary immune response phase.

Applications Reported: Flow cytometric analysis and functional blocking of CD86.
Applications Tested: Verified by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.25 µg/test (100 µL volume). Cell number may vary (10⁵–10⁸ cells/test). Antibody titration is advised for optimal performance.

Target Information

CD86 is a ligand for CTLA4 and CD28, acting as a costimulatory molecule in T-cell activation during antigen presentation. Insufficient co-stimulation can induce tolerance. CD86 is expressed by antigen-presenting cells and mediates both activation (via CD28) and inhibition (via CTLA4) of T-cell responses.

Alternative splicing produces multiple CD86 isoforms. Dysfunction in CD86 has been associated with gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.