Thermo Fisher Scientific CD8a Monoclonal Antibody (4SM15), eBioscience
Mouse CD8a 단백질을 인식하는 Rat monoclonal antibody (clone 4SM15)로, Flow Cytometry 및 IHC(P) 등 다양한 응용에 적합. Affinity chromatography로 정제되었으며, PBS buffer에 보관. 4°C 저장, 연구용 전용.
- 카탈로그번호
- 14-0808-xx (4개 옵션)
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD8a Monoclonal Antibody (4SM15), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 110 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | View 14 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 5 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Rat / IgG2a, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4SM15 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864934 |
Additional Formats
Product Specific Information
The 4SM15 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as either a heterodimer with CD8 beta (CD8αβ) or a homodimer (CD8αα). Most thymocytes and a subpopulation of mature αβ TCR T cells express CD8αβ, while γδ TCR T cells, intestinal intraepithelial lymphocytes (IELs), and dendritic cells express CD8αα. CD8 binds to MHC class I and, through association with p56lck, plays a role in T cell development and activation.
Recent studies show that the 4SM15 antibody recognizes a non-CD8a epitope in neural tissue; therefore, it is not recommended for staining mouse neural tissue. For FFPE mouse neural tissue detection, use Anti-Mouse CD8a Purified, Product #14-0195.
The 4SM15 antibody is not recommended for frozen mouse tissue IHC. For that application, use Anti-Mouse CD8a Purified, Product #14-0081-82.
Applications Reported: Flow cytometry, microscopy, and IHC of formalin-fixed paraffin-embedded (FFPE) tissue sections.
Applications Tested:
- IHC (FFPE mouse tissue): ≤5 µg/mL
- Flow cytometry (mouse splenocytes): ≤0.25 µg/test (100 µL final volume)
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family, essential for signal transduction and T cell differentiation/activation. CD8 is primarily expressed as a CD8αβ heterodimer on T cells, functioning as a co-receptor with TCR for MHC-I molecules. CD8α also forms homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells. Engagement with MHC-I/peptide complexes recruits Lck kinase, leading to cytokine production, motility, and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are critical for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.