Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (GL1), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 605
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Custom conjugation available upon request

Product Specific Information

The GL1 monoclonal antibody reacts with mouse CD86 (B7-2), an ~80 kDa surface receptor and member of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated through stimuli such as BCR complex, CD40, and cytokine receptors. It is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells.

CD86 interacts with T cell surface molecules CD28 and CD152 (CTLA-4), playing a critical role in T-B cell crosstalk, T cell costimulation, and antibody production. Its rapid upregulation upon stimulation indicates a key role during the primary immune response.

Applications Reported: Flow cytometry, immunoprecipitation, frozen tissue staining, and blocking studies (functional assays should use Functional Grade purified GL1, Product #16-0862).

Applications Tested: Tested by flow cytometric analysis of resting and activated mouse splenocytes. Recommended ≤0.5 µg/test (100 µL volume). Optimal titration is advised for specific assay conditions.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD86 is one of two ligands (along with CD80) for CTLA4 and CD28, functioning as a costimulatory molecule in T-cell activation. Insufficient co-stimulation via CD86/CD80 can lead to immune tolerance. CD86 is a type I membrane protein of the immunoglobulin superfamily, expressed on antigen-presenting cells. It binds CD28 (activating signal) and CTLA-4 (inhibitory signal) on T cells, regulating immune responses. Alternative splicing generates multiple isoforms. CD86 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.