Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (GL1), eBioscience
마우스 CD86(B7-2)을 인식하는 GL1 단클론 항체로, Flow Cytometry, IHC, IP 등 다양한 응용에 적합합니다. 0.5 µg/test로 사용 가능하며, Affinity chromatography로 정제되었습니다. 면역반응 조절 연구 및 T세포 활성화 기전 분석에 유용합니다.
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- 14-0862-8x (3개 옵션)
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 9 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 0.5 µg/test | 135 |
| ELISA | - | 1 |
| Immunoprecipitation (IP) | Assay-Dependent | 1 |
| Neutralization (Neu) | Assay-Dependent | 2 |
| Functional Assay | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GL1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467367 |
Available Conjugated Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 737
- Brilliant Violet 605
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Custom conjugation available upon request
Product Specific Information
The GL1 monoclonal antibody reacts with mouse CD86 (B7-2), an ~80 kDa surface receptor and member of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated through stimuli such as BCR complex, CD40, and cytokine receptors. It is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells.
CD86 interacts with T cell surface molecules CD28 and CD152 (CTLA-4), playing a critical role in T-B cell crosstalk, T cell costimulation, and antibody production. Its rapid upregulation upon stimulation indicates a key role during the primary immune response.
Applications Reported: Flow cytometry, immunoprecipitation, frozen tissue staining, and blocking studies (functional assays should use Functional Grade purified GL1, Product #16-0862).
Applications Tested: Tested by flow cytometric analysis of resting and activated mouse splenocytes. Recommended ≤0.5 µg/test (100 µL volume). Optimal titration is advised for specific assay conditions.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD86 is one of two ligands (along with CD80) for CTLA4 and CD28, functioning as a costimulatory molecule in T-cell activation. Insufficient co-stimulation via CD86/CD80 can lead to immune tolerance. CD86 is a type I membrane protein of the immunoglobulin superfamily, expressed on antigen-presenting cells. It binds CD28 (activating signal) and CTLA-4 (inhibitory signal) on T cells, regulating immune responses. Alternative splicing generates multiple isoforms. CD86 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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