Thermo Fisher Scientific CD45RO Monoclonal Antibody (UCHL1), APC, eBioscience

Thermo Fisher Scientific CD45RO Monoclonal Antibody (UCHL1), APC, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.03 µg)/test
Publications: View 16 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 647
• Alexa Fluor Plus 750
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant Violet 650
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes, and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is commonly used to discriminate subsets of peripheral T cells.

Applications Reported:
This UCHL1 antibody is reported for use in flow cytometric analysis.

Applications Tested:
This antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. It can be used at 5 µL (0.03 µg) per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test).

Excitation/Emission: 633–647 nm / 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of the receptor-type protein tyrosine phosphatase CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, acting as a regulator of Src-family kinases. It promotes cell survival by modulating integrin-mediated signaling and is involved in DNA fragmentation during apoptosis.

CD45 isoforms differ in their extracellular domains but share identical transmembrane and cytoplasmic regions. Their expression depends on cell type and physiological state. CD45RO is expressed on macrophages, CD8⁺ T cells, activated T cells, and myeloma cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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