Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), APC, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC-eFluor 780
• Biotin
• Brilliant UV (395–805 series)
• Brilliant Violet (421–786 series)
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor (Blue, Red, Violet, Yellow series)
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright (436–780 series)
• Custom conjugation available

Product Specific Information

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. The cytoplasmic portion of CD45 exhibits tyrosine phosphatase activity and plays a crucial role in lymphocyte activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse bone marrow cells and splenocytes (≤0.125 µg/test in 100 µL final volume)
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, constituting ~10% of lymphocyte surface proteins. It is absent from non-hematopoietic cells and tissues. CD45 regulates Src-family kinases, lymphocyte development, and antigen signaling. Isoforms arise from alternative splicing and differ in extracellular domains. CD45 is essential for T- and B-cell receptor signaling and influences cytokine receptor pathways, integrin-mediated survival signals, and apoptotic regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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