Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), APC, eBioscience
Mouse CD45 인식 단일클론 항체(30-F11)로 모든 CD45 동형체에 반응하며, APC 형광표지로 Flow Cytometry에 최적화됨. 0.125 µg/test로 사용 가능하며, 633–647 nm 레이저에서 여기, 660 nm 방출. 4°C 암소 보관, Affinity chromatography로 정제됨.
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- 17-0451-8x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), APC, eBioscience
Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 publications |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | 150 publications |
| Immunoprecipitation (IP) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-F11 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469392 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC-eFluor 780
- Biotin
- Brilliant UV (395–805 series)
- Brilliant Violet (421–786 series)
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor (Blue, Red, Violet, Yellow series)
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright (436–780 series)
- Custom conjugation available
Product Specific Information
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. The cytoplasmic portion of CD45 exhibits tyrosine phosphatase activity and plays a crucial role in lymphocyte activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of mouse bone marrow cells and splenocytes (≤0.125 µg/test in 100 µL final volume)
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, constituting ~10% of lymphocyte surface proteins. It is absent from non-hematopoietic cells and tissues. CD45 regulates Src-family kinases, lymphocyte development, and antigen signaling. Isoforms arise from alternative splicing and differ in extracellular domains. CD45 is essential for T- and B-cell receptor signaling and influences cytokine receptor pathways, integrin-mediated survival signals, and apoptotic regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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