Thermo Fisher Scientific NEFL Recombinant Rabbit Monoclonal Antibody (3L9Y10)
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NEFL 단백질을 인식하는 재조합 토끼 단클론 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합하며, 고순도 Affinity Chromatography 정제 및 안정적인 PBS/glycerol 버퍼로 제공됩니다.
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Thermo Fisher Scientific NEFL Recombinant Rabbit Monoclonal Antibody (3L9Y10)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:400,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:800 |
| ELISA | 1 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3L9Y10 |
| Immunogen | Recombinant fusion protein containing amino acids 60–250 of human Neurofilament L (NP_006149.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2911893 |
Product Specific Information
- Positive test controls: SH-SY5Y, Mouse brain, Rat brain
- Immunogen sequence:
SSGSLMPSLE NLDLSQVAAI SNDLKSIRTQ EKAQLQDLND RFASFIERVH ELEQQNKVLE AELLVLRQKH SEPSRFRALY EQEIRDLRLA AEDATNEKQA LQGEREGLEE TLRNLQARYE EEVLSREDAE GRLMEARKGA DEAALARAEL EKRIDSLMDE ISFLKKVHEE EIAELQAQIQ YAQISVEMDV T
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons, composed mainly of three major subunits: NF-L, NF-M, and NF-H.
- NF-L: Light subunit (~70 kDa)
- NF-M: Medium subunit
- NF-H: Heavy subunit (180–220 kDa, species-dependent)
These proteins are essential for maintaining neuronal caliber and are developmentally regulated.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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