Thermo Fisher Scientific BAX Recombinant Rabbit Monoclonal Antibody (2F7I0)
Recombinant rabbit monoclonal antibody targeting human BAX protein. Validated for WB, ICC/IF, ELISA, and IP. High specificity and affinity-purified for reliable apoptosis-related research. Supplied in liquid form, suitable for multiple species reactivity.
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Thermo Fisher Scientific BAX Recombinant Rabbit Monoclonal Antibody (2F7I0)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:2,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2F7I0 |
| Immunogen | Synthetic peptide corresponding to amino acids 10–70 of human Bax (NP_620116.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2911891 |
Product Specific Information
Positive test controls include: 293T, HT-1080 cells.
Target localization: Cytoplasm, Mitochondrion membrane, Single-pass membrane protein.
Immunogen sequence:
GGGPTSSEQI MKTGALLLQG FIQDRAGRMG GEAPELALDP VPQDASTKKL SECLKRIGDE L
Target Information
BAX is a member of the Bcl-2 family, playing a crucial role in apoptosis regulation.
While Bcl-2 acts as an anti-apoptotic protein, BAX promotes apoptosis.
Apoptosis regulation involves homo- and heterodimerization of BAX and Bcl-2 isoforms.
BAX gene encodes isoforms Bax alpha (21 kDa) and Bax beta (24 kDa).
Both contain BH1, BH2, and BH3 domains; Bax beta lacks a hydrophobic transmembrane domain.
Overexpression of BAX promotes cell death through dimerization with BCL-2-related proteins.
Usage Note
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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