Thermo Fisher Scientific MEK1 N-Term Polyclonal Antibody, FITC
FITC로 표지된 MEK1 N-말단 특이 토끼 다클론 항체로, 인간, 마우스, 랫트에 반응합니다. Western blot 및 ELISA에 사용 가능하며, 형광 특성은 498/517 nm입니다. 친화 크로마토그래피로 정제되어 높은 특이성과 안정성을 제공합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH. |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This fluorescein-conjugated, affinity-purified antibody is directed against human MEK1 protein. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by various growth factors, cytokines, membrane depolarization, and calcium influx. MEK1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is involved in stress response signaling, apoptosis, and cytokine-induced proliferation. Mutations in the gene can lead to cardiofaciocutaneous syndrome.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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