
Thermo Fisher Scientific Collagen Type VI Polyclonal Antibody, FITC
FITC로 표지된 Collagen Type VI 폴리클로날 항체로, WB, IHC, ELISA, IP 등 다양한 응용에 사용 가능. 인간 및 소 콜라겐 VI에 반응하며, 다른 콜라겐 타입과 교차반응이 거의 없음. 형광 특성은 498/517 nm, 친화 크로마토그래피로 정제됨.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Collagen Type VI from human and bovine placenta |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles; store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- Restore with 0.05 mL of deionized water or equivalent.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
- Expiration date: one year from date of restoration.
Typically negligible cross-reactivity against other collagen types was detected by ELISA using purified standards.
This antibody reacts with most mammalian Type VI collagens and shows negligible cross-reactivity with Type I, II, III, IV, and V collagens.
Non-specific cross-reaction with other human serum or extracellular matrix proteins is negligible.
Target Information
The COL gene family encodes various collagen chains, including fibril-forming interstitial collagens (types I, II, III, V) and basement membrane collagen (type IV).
Collagens are fibrous extracellular matrix proteins with high tensile strength and are major components of connective tissues such as tendons and cartilage.
They contain a triple helix domain and often self-associate laterally to form complex tissue structures.
Some collagens also participate in cell adhesion, maintaining tissue architecture and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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