Thermo Fisher Scientific GST Polyclonal Antibody, FITC
GST 태그 단백질 검출용 FITC 결합 폴리클로날 항체. Western blot, ICC/IF, ELISA, IP 등 다양한 어플리케이션에 사용 가능. Schistosoma japonicum 유래 GST를 면역원으로 제작. 고순도 친화성 크로마토그래피 정제. 연구용으로만 사용.
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Thermo Fisher Scientific GST Polyclonal Antibody, FITC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:60,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full-length GST isolated from Schistosoma japonicum |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, as well as purified and partially purified Glutathione-S-Transferase (Schistosoma japonicum). Cross-reactivity against GST from other sources may occur but has not been specifically determined.
Target Information
GST (Glutathione S-transferase) is a 26 kDa protein found in both eukaryotes and prokaryotes, catalyzing a variety of reactions. GST is used to create GST gene fusion systems, allowing purification of GST-fusion proteins via glutathione affinity. Fusion proteins are essential tools for studying protein-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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