Thermo Fisher Scientific GFP Polyclonal Antibody, FITC

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
• Centrifuge product if not completely clear after standing at room temperature.
• Product is stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Fluorescein, and purified or partially purified Green Fluorescent Protein (Aequorea victoria) Serum.
No reaction was observed against Human, Mouse, and Rat Serum Proteins.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.

As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments. GFP technology has provided new insights into cellular physiology.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without requiring additional proteins or cofactors.

GFP fluorescence is stable, species-independent, and suitable for various applications including:

• Monitoring gene expression and protein localization
• Studying protein-protein interactions (e.g., yeast two-hybrid system)
• Measuring distances between proteins in FRET experiments

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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