Thermo Fisher Scientific GFP Polyclonal Antibody, FITC
FITC로 표지된 GFP 폴리클로날 항체로, Western blot, IHC, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. Aequorea victoria 유래 GFP를 면역원으로 사용하며, 높은 특이성과 형광 안정성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 1:20,000–1:40,000 |
| Immunoprecipitation (IP) | 1:10,000–1:25,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Green Fluorescent Protein (GFP) produced by immunizing GFP-containing fusion protein corresponding to the full-length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. |
| Conjugate | FITC (Fluorescein isothiocyanate) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Fluorescein, and purified or partially purified Green Fluorescent Protein (Aequorea victoria) Serum.
No reaction was observed against Human, Mouse, and Rat Serum Proteins.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments. GFP technology has provided new insights into cellular physiology.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without requiring additional proteins or cofactors.
GFP fluorescence is stable, species-independent, and suitable for various applications including:
- Monitoring gene expression and protein localization
- Studying protein-protein interactions (e.g., yeast two-hybrid system)
- Measuring distances between proteins in FRET experiments
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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