Thermo Fisher Scientific Goat anti-Human IgG Fc Secondary Antibody, PE, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

1 µg/test

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

PE PE PE

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Human IgG Fc/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Biotin/host/Goat/)
• [FITC](/antibody/secondary/product-name/Human IgG Fc/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Human IgG Fc/conjugates/HRP/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Human IgG Fc/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465926

Target

IgG Fc

Antibody Form

Whole Antibody

Product Specific Information

Description: This goat antibody reacts with the Fc fragment of heavy chains on human IgG but notwith the light chains on most human immunoglobulins. No antibody was detected against human IgM or IgA, or against nonimmunoglobulinserum proteins. This antibody has been tested to ensure minimalcross-reaction with bovine, horse, and mouse serum proteins, but the antibody may cross-react with immunoglobulins fromother species.

Applications Reported: PE goat anti-Human IgG (Fc gamma Fragment Specific) has been reported for use in flow cytometric analysis.

Applications Tested: PE goat anti-Human IgG (Fc gamma Fragment Specific) has been tested by flow cytometric analysis to detect purified human Fc gamma-tagged recombinant proteins. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.