Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience

Applications

Tested Dilution

• Flow Cytometry (Flow): Assay-Dependent
• Control (Ctrl): Assay-Dependent

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• PE-Cyanine5, 5.5, 7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The eBio299Arm monoclonal antibody serves as an isotype control immunoglobulin.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes and normal human peripheral blood
• Recommended Use: Use at the same concentration as the experimental antibody
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green lasers
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Isotype control antibodies help differentiate non-specific background signal from specific antibody signal by lacking specificity to any target antigen.
They are essential for:

• Identifying background staining caused by Fc receptor binding or nonspecific interactions
• Reducing false positives in flow cytometry, ChIP, IHC, and gel shift assays
• Matching species and isotype with the primary antibody for accurate comparison
• Ensuring consistent labeling when using directly conjugated antibodies

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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