Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience
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Armenian hamster 유래 단클론 IgG 동형 대조 항체로, Flow Cytometry용 PE 형광 표지 제품입니다. 비특이적 배경 신호 구분에 사용되며, 565/576 nm 형광 특성을 가집니다. 4°C 보관, 동결 금지.
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Applications
Tested Dilution
- Flow Cytometry (Flow): Assay-Dependent
- Control (Ctrl): Assay-Dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBio299Arm |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_470073 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- PE-Cyanine5, 5.5, 7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780
- Custom conjugation available
Product Specific Information
The eBio299Arm monoclonal antibody serves as an isotype control immunoglobulin.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes and normal human peripheral blood
- Recommended Use: Use at the same concentration as the experimental antibody
- Excitation: 488–561 nm
- Emission: 578 nm
- Laser Compatibility: Blue, Green, Yellow-Green lasers
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Isotype control antibodies help differentiate non-specific background signal from specific antibody signal by lacking specificity to any target antigen.
They are essential for:
- Identifying background staining caused by Fc receptor binding or nonspecific interactions
- Reducing false positives in flow cytometry, ChIP, IHC, and gel shift assays
- Matching species and isotype with the primary antibody for accurate comparison
- Ensuring consistent labeling when using directly conjugated antibodies
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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