Thermo Fisher Scientific Mouse anti-Rat IgG2a Secondary Antibody, PE, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.06 µg/test

Product Specifications

Additional Available Formats

• eFluor 570
• eFluor 615
• eFluor 660
• FITC
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and is used as a secondary reagent in flow cytometry and microscopy. It does not cross-react with other rat isotypes or mouse antibodies.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometry using rat IgG2a primary antibody
• Recommended Use: ≤ 0.06 µg per 100 µL test volume (10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Anti-Rat secondary antibodies are affinity-purified reagents with high specificity for rat immunoglobulins. They are widely used for detection, sorting, and purification of target antibodies. Secondary antibodies enhance sensitivity through signal amplification, as multiple secondaries can bind a single primary antibody. These reagents are typically generated by immunizing host animals with pooled rat immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for optimal performance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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