Thermo Fisher Scientific GFP Polyclonal Antibody, HRP
GFP 단백질을 인식하는 HRP 결합 폴리클로날 항체로, Western blot, IHC, ELISA, IP 등 다양한 분석에 사용 가능. 친화 크로마토그래피로 정제된 고순도 항체이며, 안정적인 형광 단백질 검출에 적합. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:2,000–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Green Fluorescent Protein (GFP) produced by immunizing GFP-containing fusion protein corresponding to the full-length amino acid sequence (246aa) derived from Aequorea victoria |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay Information:
Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Peroxidase, and purified or partially purified GFP (Aequorea victoria) Serum.
No reaction observed against Human, Mouse, and Rat Serum Proteins.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression and as a protein tag in both eukaryotic and prokaryotic organisms.
As a fusion tag, GFP enables localization studies, movement tracking, and research on subcellular compartment dynamics. GFP technology provides insights into physiological activities of living cells.
- GFP is a 27 kDa monomeric protein forming a fluorescent pigment autocatalytically.
- Absorbs blue light (max 395 nm) and emits green light (peak 508 nm).
- Fluorescence is stable, species-independent, and suitable for various applications.
- Commonly used for monitoring gene expression, protein localization, protein-protein interactions (yeast two-hybrid), and FRET experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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