Thermo Fisher Scientific GST Polyclonal Antibody, HRP
GST 태그 단백질 검출용 HRP 결합 폴리클로날 항체. Western blot, IHC, ELISA, IP에 적합. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. Schistosoma japonicum 유래 GST를 면역원으로 제작. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:1,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full length GST isolated from Schistosoma japonicum |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Peroxidase, as well as purified and partially purified Glutathione-S-Transferase (Schistosoma japonicum).
Cross-reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Target Information
GST (Glutathione S-transferase) is a 26 kDa protein present in both eukaryotes and prokaryotes, catalyzing various reactions. It is commonly used to create GST gene fusion systems, enabling purification of fusion proteins via glutathione affinity.
Fusion proteins are valuable tools for studying direct protein–protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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