Thermo Fisher Scientific SMAD5 Polyclonal Antibody
SMAD5 단백질 검출용 Rabbit Polyclonal Antibody로 Western blot, ICC/IF, ChIP assay에 적합. 인간에 반응하며 Mouse, Rat 등에서도 예측 반응성 있음. 항원 친화 크로마토그래피로 정제되고 PBS 기반 용액 형태로 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SMAD5 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736631 |
Product Specific Information
- Positive Control: Target recombinant protein
- Predicted Reactivity: Mouse (97%), Rat (99%), Chicken (93%), Rhesus Monkey (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SMADs are members of the MAD-related family of molecules. These intracellular proteins are essential components in the signaling pathways of serine/threonine kinase receptors of the transforming growth factor beta (TGF-β) superfamily.
SMADs are categorized as:
- R-SMADs: SMAD1, 2, 5, 8, 9
- Co-SMAD: SMAD4
- I-SMADs: SMAD6, 7
SMAD1, 5, 8, and 9 share high sequence homology, and antibodies recognizing common sequences are available. SMAD8 and SMAD9 are often used interchangeably in literature.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
Usage Restriction
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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