Thermo Fisher Scientific Aurora B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

-

ELISA (ELISA)

1:2,000-1:10,000

-

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Non-human primate, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 227-238 of human Aurora-B kinase if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Aurora B,uniProtId:Q96GD4-1,ncbiNodeId:9606,antigenRange:227-238,antigenLength:344,antigenImageFileName:PA1-86828_Aurora_B_Q96GD4-1_Rabbit.svg,antigenImageFileNamePDP:PA1-86828_Aurora_B_Q96GD4-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2061628

Product Specific Information

Predicted to react with bovine, chimpanzee, dog, mouse, pig and rat based on sequence homology.

In Western blot, this antibody detects a band at ~39kDa in HeLa cell lysate.

Rabbit anti Human Aurora-B kinase antibody detects human Aurora-B kinase (AurB), a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC).

Target Information

The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.