Thermo Fisher Scientific TIM-1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0-2.0 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

16 amino acid peptide sequence near the center of human TIM-1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2116449

Product Specific Information

In Western blot, this antibody detects a band at ~49kDa in human uterus tissue lysate.

Rabbit anti TIM-1 antibody detects T cell immunoglobulin mucin-1 (TIM-1), which was first identified as the receptor used by hepatitis A for viral entry, it is also known as the hepatitis A virus cellular receptor- HAVCR (Curtiss and Colgan 2007), TIM -1 also acts as a receptor for other viruses, including Dengue, Ebola and Marburg (Kondratowicz et al. 2011).

Target Information

CD365 also known as T-cell immunoglobulin and mucin domain 1 (Tim-1) is a type 1 surface glycoprotein that consists of extracellular N-terminal immunoglobulin V-like domain and a mucin domain, and a short cytoplasmic tail. The endogenous ligand for CD365 is Tim-4. In addition, CD365 is known to be a cellular receptor or co-receptor for a number of viruses including hepatitis A and C viruses, Ebola virus, Marburg virus, Dengue virus, Zika virus, West Nile virus, and Japanese encephalitis virus. The direct binding of the viruses by CD365 occurs via phosphatildyl-serine exposed on viral envelopes. Polymorphisms within human CD365 gene (HAVCR1) are associated with susceptibility to infection and the disease severity. CD365 has also been implicated in the T cell co-stimulation. CD365 is primarily expressed in epithelial cells. It is also highly expressed in A549, 769-P and Caco-2 and cell lines. CD365 exists also as a soluble protein generated by metalloproteinase mediated proteolytic cleavage of the surface protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.