Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), NovaFluor Yellow 700, eBioscience

Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), NovaFluor Yellow 700, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.05 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 711
• Brilliant Violet 786
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700 / 725 / 755
• NovaFluor Yellow 610 / 690 / 755
• PE / PE-Cyanine5 / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated through various stimuli including BCR, CD40, and cytokine receptors.

CD86 interacts with CD28 and CTLA-4 on T cells, playing a key role in T-B cell crosstalk, T cell costimulation, and antibody production. Its upregulation kinetics support its major role in the primary immune response phase.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry on human peripheral blood cells. Use 5 µL (0.05 µg)/test in 100 µL final volume. Cell number range: 10⁵–10⁸ cells/test.

Note: NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Required for optimal staining with NovaFluor conjugates
• Add 5 µL per sample (regardless of number of NovaFluors)
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells)

NovaFluor conjugates utilize Phiton technology with engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 552 nm
Emission: 700 nm
Laser: 561 nm (Yellow)

Target Information

CD86 is a ligand for CTLA4 and CD28, acting as a costimulatory molecule in T-cell activation during antigen presentation. Inadequate co-stimulation via CD86/CD80 can induce tolerance. CD86 is a type I membrane protein of the immunoglobulin superfamily, expressed by antigen-presenting cells.

Binding with CD28 provides activation signals, while binding with CTLA-4 inhibits T-cell activation. Alternative splicing produces multiple isoforms of CD86.

Associated Diseases: Gallbladder squamous cell carcinoma, myocarditis.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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